BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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F10S1 and Bacillus sp.

Biodiversity Heritage Library

The increased levels of phenols and phenoxides may be directly related to the accumulation of end compounds produced by the degradation of resins and asphaltenes. No statistically significant differences were found for this parameter among the examined strains, although the increased proportion of carboxyl and hydroxyl groups demonstrates the presence of biological oxidation processes.

Publications reporting the presence of this genus in hydrocarbon-contaminated ecosystems and describing its hydrocarbon-degrading abilities have appeared in the literature from the early nineties [17], although its numbers baacterias increased as of late [19, 24, 25, degraxadoras, 32].

A microbiological survey of the western Cuban continental shelf found this species in both northern and southern locations [27]. An analysis of these results leads us to suppose that linear chains up to 30 carbon atoms long and some low molecular weight aromatic compounds were degraded during the first 10 to 15 days.

After concluding the three subculture cycles, 0. Since d physico-chemical properties make them toxic compounds for the growth of most bacteria [44, 50], it is assumed that the degradation of aromatic bactdrias does not start until saturated hydrocarbons have been used up. Molecular characterization of a toluene-degrading methanogenic consortium. Depth-resolved quantification of anaerobic degradaforas degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume.

The obtained isolates were then subjected to selection in Bushnell-Haas medium supplemented with a heavy crude oil, selecting three strains able to degrade this hydrocarbon mixture within a period of seven days. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants.


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All spectra were processed using the Omnic v5. Taxonomy of aerobic marine eubacteria. The concentration of associated hydroxyl groups OH -cm -1 increased for all treatments, as did that of carboxylic groups cm Phenotypic characterization of the strains The strains were characterized phenotypically using previously described morphological, physiological and biochemical tests [], using previously defined criteria to describe culture characteristics [13].

However, and despite the higher propensity of n-alkanes for oxidation [35, 49], no differences in biodegradation percentages were detected between saturated hydrocarbons, asphaltenes and monocyclic aromatic hydrocarbons after 45 days.

Since hydrocarbon determinations were performed solely at the end of the study day 45no data are available to evaluate the biodegradation process in earlier time points. In both protocols deggradadoras marine bacteria isolation plates were periodically examined after 24 h of growth under a stereoscope, streaking onto separate plates all the colonies appearing during the seven day incubation period.

Universidad de La Habana; Viewed as a whole, the latter possess the necessary genetic information to produce all the enzymes required to completely degrade complex hydrocarbon mixtures in damaged areas [4, 5]. Recent advances bactterias petroleum microbiology. Universidad de Oriente; Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments.

Biosurfactant-enhanced degradation of residual hydrocarbons from slip bilge wastes. Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation.

Each chart depicts both the profile of the strain under examination and that of the standard sample. Strain F10S1 lowered the concentration of all fractions in comparison with the control.

The organic phase of the samples was extracted with 45 mL of HPLC-grade dichloromethane three extractions with a volume of 15 mL each using the liquid-liquid method for 30 min in a separating funnel, filtering the obtained organic extract through anhydrous reagent grade sodium sulfate. Las potencialidades biodegradadoras de estos microorganismos en la limpieza de costas marinas han generado nuevos estudios.

Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel. Strain isolation The primary selection process employed two different discrimination protocols, denominated here protocol A modified from [8] and B modified from [9]. Environmental biotechnology techniques have been readily used to remediate ecological disasters ddegradadoras by large oil spills, such as those caused by the collision of tanker Exxon Valdez detradadoras Bligh Reef at Prince William Sound, spilling some 50 tons of crude oil in March [6], and the sinking of Prestigewhich spilled 63 tons of crude and ended up affecting 1 km of French and Spanish coastlines in November [7].


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Usually found at low concentrations in non-contaminated degradaeoras, their populations bloom in chronically contaminated environments [1]. Two degradaxoras the isolated strains belonged to the Pseudomonas genus.

Finer taxonomic classification to the level of species was not pursued since, despite the existence of taxonomic identification schemes for microorganisms from marine ecosystems [12, 15], successfully identifying recently described genera and species of marine bacteria requires the application of molecular methods [16].

It has been shown that strain WW1 of Alcaligenes denitrificans can degrade four-ring polyaromatic hydrocarbons [30]. Biodegradation of crude oil contaminating marine shorelines and freshwater wetlands. Each of the images shown in the figure contains the profiles of the abiotic control and that of the strain under examination, for a number of different carbon chain lengths. Some species of this genus have been isolated from marine environments contaminated with hydrocarbons [5, 17, 28].

Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the petroldo diversity of hydrocarbon-degrading representatives from this group, but to petrokeo ability to use xenobiotic compounds as carbon source in pure cultures [3]. Degradadoraz tolerance was estimated by seeding the test strains in marine bacteria isolation medium where seawater was replaced by distilled water and sodium chloride NaCl concentration was set at 0, 0.

We were unable to find previous mentions in the literature of the presence of these microorganisms in hydrocarbon-contaminated environments. Online J Biol Sci.